[目的]测定不同方法提取的白花丹鲜叶中白花丹醌的含量并加以比较,优选出最佳提取溶剂.[方法]采用索氏提取法,选用不同的溶剂提取白花丹鲜叶中的白花丹醌,并通过HPIC测定其含量,从而确定最佳提取溶剂.[结果]测得用氯仿、石油醚(Ⅱ)、浓度95％乙醇、无水甲醇提取白花丹醌的含量依次为0.511％ 、0.341％、0.233％和0.191％；用氯仿提取的白花丹醌含量(提取率)最高.[结论]不同方法提取的白花丹鲜叶中白花丹醌的含量差异明显.%[Objective] To determine and compare the contents of plumbagin in the leaves of Plumbago zeylanica L. By different extraction methods, and to select the optimal extraction solvent. [ Method ] Soxhlet extraction method was adopted; plumbagin in the leaves of P. Zeylanica was extracted by different solvents. Its content was detected by HPLC, so as to select the optimal extraction solvent. [ Result] Contents of plumbagin extracted by chloroform, petroleum ether ( Ⅱ), 95% ethanol and anhydrous methanol were 0. 511% , 0. 341% , 0. 233% and 0.191%, respectively. Content of plumbagin in P. Zeylanica extracted by chloroform was the highest. [Conclusion] There were significant differences in the content of plumbagin in P. Zeylanica fresh leaves by different extraction methods.
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