膜超滤分离美洲大蠊抗肿瘤活性部位小分子肽的研究

摘要

[目的]建立美洲大蠊抗肿瘤活性部位小分子肽的超滤膜分离工艺.[方法]采用中空纤维超滤膜(3 kDa)对美洲大蠊抗肿瘤活性部位小分子肽进行分离,通过单因素试验考察了转速、浓缩比、pH及操作时间对分离效果的影响,并在单因素试验的基础上通过正交试验优化了分离工艺条件.[结果]超滤膜分离美洲大蠊抗肿瘤活性部位小分子肽的最佳工艺条件为转速4 500 r/min、浓缩比30％以及pH 11,其中转速是最主要的影响因素.[结论]为膜超滤分离美洲大蠊抗肿瘤活性部位小分子肽的工业化奠定了基础.%[Objective] The paper was to establish ultrafiltration membrane technology for separation of antitumor active plypeptides from Periplaneta americana. [ Method] Hollow fiber ultrafiltration membrane(3 kDa) was used to separate antitumor active plypeptides from P. Amer-icana. Through single factor test,the impact of rotation speed,concentration ratio,pH and operation time on separation effort was studied,and the separation technology conditions were further optimized through orthogonal test. [ Result ] The best technology conditions for separation of antitumor active plypeptides from P. Americana were as follows: rotation speed 4500 r/min, concentration ratio 30% ,pH 11, and the rotation speed was the major influencing factor. [ Conclusion] The study laid foundation for industrialization of ultrafiltration membrane separation of antitumor active plypeptides from P. Americana.