[目的]研究顶果木离体培养最佳方法.[方法]以顶果木种子获得的无菌苗进行离体培养,通过诱导丛生芽的发生,获得再生植株.[结果]采用MS+ 6-BA 0.8～3.0 mg/L+ NAA 0.2～0.5 mg/L培养基、改良MS+ 6-BA 0.2～1.2 mg/L+ NAA 0.18～0.2 mg/L培养基均能保持无菌苗的生长,其中改良MS +6-BA 0.2 mg/L+ NAA 0.18 mg/L培养基最适宜无菌苗生长,但培养40d后,未见芽苗分化;将无菌苗转入改良MS +6-BA 0.5～ 1.2 mg/L+ NAA 0.2 mg/L培养基中培养,均能诱导芽的分化和增殖,培养20d后,芽繁殖系数可达1.72 ～ 2.30,其中改良MS+ 6-BA 1.2 mg/L+ NAA 0.2 mg/L培养基芽繁殖系数达2.30,总体芽苗生长情况差异不明显.[结论]改良MS+ 6-BA 1.2 mg/L+ NAA 0.2 mg/L培养基为诱导顶果木最适培养基.%[Objective] The aim of the study was to seek the best method on tissue culture of Acrocarpus fraxinifoliu. [Method] The multi-buds induction and regeneration system of Acrocarpus fraxinifolius from seed in vitro was preliminary investigated. [ Result] The results showed that the MS+6-BA0. 8 -3.0 mg/L + NAA 0. 2-0.5 mg/L culture medium,modified MS+ 6-BA 0.2 - 1.2 mg/L + NAA 0. 18-0.2 mg/L culture medium could maintain seedling growth, which modified MS + 6-BA 0. 2 mg/L + NAA 0.18 mg/L culture medium was the most suitable for seedling growth, but the bud differentiation were not happened after 40 days. The modified MS + 6-BA 0.5 -1.2 mg/L + NAA 0.2 mg/L culture medium could induce bud differentiation and proliferation. The propagation coefficient of the bud could reach 1. 72 -2. 30 after 20 days, which the propagation coefficient was 2. 30 that culture in modified MS +6-BA 1. 2 mg/L + NAA 0. 2 mg/L medium, the overall shoots growth were not significant. [ Conclusion] The modified MS +6-BA 1.2 mg/L + NAA 0.2 mg/L culture medium was the most suitable for tissue culture of Acrocarpus fraxinifoliu.
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