[Objective] Ephedra callus induced with Ephedra explant were cultured in suspension culture. [Method] The conditions needed in callus induction, callus generation and suspension culture of Ephedrae sinica Stapf cotyledon were studied. [Result] The results showed that the best medium for callus induction was MS + 2.0 mg/L 2,4-D + 1.0 mg/L 6-BA. The best media of callus generation was MS + 1. 5 mg/L 2,4-D + 1.5 mg/L 6-BA. The best media of suspension culture was 2.0 mg/L 2,4-D + 1.5 mg/L 6-BA + CH 300 mg/L, increased amount of dry weight was 0. 80 g. [Conclusion] The condition of Ephedrae simca Stapf suspension culture was preliminary established, which will lay a foundation for the expanding culture and extracting active ingredient of Ephedrae sinica Stapf cell.%[目的]对草麻黄的子叶诱导出的愈伤组织,进行悬浮培养.[方法]采用组织培养的方法进行了愈伤组织诱导、愈伤组织继代和悬浮培养研究.[结果]MS+2.0mg/L2,4-D+ 1.0 mg/L 6-BA为诱导子叶形成愈伤组织的理想培养基；MS+ 1.5 mg/L2,4-D+ 1.5 mg/L6-BA是愈伤组织的适宜继代培养基；2.0mg/L2,4-D+ 1.5 mg/L 6-BA+ 300 mg/L水解酪蛋白(CH)是愈伤组织悬浮培养的适宜培养基,愈伤组织干重增加量为0.80 9.[结论]初步选择出草麻黄愈伤组织细胞的悬浮培养条件,为麻黄细胞扩大培养及有效成分提取奠定基础.
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