[Objective] The research aimed to study the correlation between the isozyme phenotype of isocitrate dehydrogenase-1 (IDH-1)and the biological characters of Scophthalmus maximus L.. [Method] Using isozyme starch gel electrophoresis and specific dying method,the expression of IDH-1 in 60 S. Maximus artificially cultured was studied. The phenotype of IDH-1 and biological indices of S. Maximus were detected and the correlation between the isozyme phenotype and the biological indices was analyzed. [ Result ] 2 genotypes of IDH-1 (A1 and A2) of S. Maximus were obtained by using the isozyme electrophoresis and 53 individuals showed genotype A1 and 7 individuals showed genotype A2. In the population of 116-day-old and 165-day-old S. Maximus,the average values of body weight, body length and body height of individuals with genotype A2 were all higher than that with genotype A,. The body height of 116-day-old S. Maximus with genotype A2 and the body weight and body height of 165-day-old S. Maximus with genotype A2 were obviously higher than that with genotype A, ,with extremely significant difference (P< 0.01). [Conclusion] The research provided references for further studies on the marker-assisted breeding of S. Maximus.%[目的]研究大菱鲆IDH-1(异柠檬酸脱氢酶-基因座位1)同工酶表型与生物学性状的关系.[方法]采用同工酶水平切片淀粉凝胶电泳及特异性染色方法,研究了60尾人工养殖大菱鲆IDH-1的表达,并对其IDH-1表型以及生物学指标进行了检测,分析IDH-1同工酶表型与生物学指标的关系.[结果]在大菱鲆同工酶电泳中得到2种基因型A1和A2,其中基因型A153尾,基因型A2 7尾.在116日龄和165日龄的大菱鲆群体中,基因型为A2的大菱鲆个体的体重、体长和体高的平均值均高于A1个体.A2基因型的116日龄个体的体高、165日龄个体的体重和体高,均明显高于A1基因型,差异极显著(P＜0.01).[结论]该研究为进一步研究大菱鲆的标记辅助育种提供了参考.
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