柳叶中黄酮醇苷元槲皮素的含量测定

摘要

[Objective] The aim was to develop a method of RP-HPLC for determination of flavnoid aglycones - quercitin in willow leaf,and to discover its content. [Method] Willow leaves were collected in the middle of April. The flavonoids in the leaves were extracted by successive extraction with methanol. The extracts were acid-hydrolyzed before being tested with RP-HPLC. The condition of RP-HPLC was: Diamonsil C18 column (150 mm×4.6 mm,5 μm); concentration of mobile phase was 0.07% ; phosphoric acid-water-free ethanol (50:50,V/V); mobile speed was 1.0 ml/min; tested wave length 260 nm; sampling amount 10 μl; flexibility 0.000 1 AUFS; column temperature was indoor temperature (20 ℃). [ Result] The constituent of flavnoid aglycones in willow leaves was mainly quercitin and it consisted of 0.36% -0.74% flavnoid aglycones. [Conclusion] The method of RP-HPLC for determination of quercitin in willow leaf provided reference for the effective use of willow.%[目的]测定柳叶中黄酮醇苷元成分槲皮素的含量.[方法]于4月中旬采摘柳叶嫩叶,用无水甲醇连续提取,将提取液用盐酸水解后采用高效液相色谱法测定槲皮素的含量.色谱条件为:色谱柱为Diamonsil C18柱(150 mm×4.6 mm,5μm),流动相为浓度0.07％磷酸-无水甲醇(50:50,V/V)；流速为1.0 ml/min;检测波长为260 nm；进样量为10μl；灵敏度为0.000 1 AUFS；柱温为室温(20℃).[结果]柳叶嫩叶中黄酮醇苷元以槲皮素为主,含量在0.36％ ～0.74％.[结论]柳叶中槲皮素含量测定方法的建立,为有效利用现有柳资源提供理论依据.