四唑染色法测定厚朴种子生活力的条件研究

摘要

[Objective] To study on rapid determination method of seed viability of Magnolia officinalis Rehd.et Wils.[Method] Using TTC method and setting different TTC concentration,dyeing temperature and dyeing time,the suitable conditions of Magnolia officinalis seed viability determination were studied.[Result] The optimal determination conditions for the viability of Magnolia offinalis seed is that staining for 50min in 55 ℃ water bath with 0.5％ TTC solution.[Conclusion] This is an effective method to be used to determine Magnolia officinalis seed viability.%[目的]探讨厚朴种子生活力的快速测定方法.[方法]采用四唑染色法(TTC法),设置不同的TTC浓度、染色温度和时间,研究厚朴种子生活力测定的适宜条件.[结果]TTC法测定厚朴种子生活力的适宜条件为:采用浓度0.2％的TTC溶液,在55℃的水浴中染色50 min.[结论]该法可作为厚朴种子生活力测定的有效方法.