七叶一枝花种子萌发和组织培养研究

摘要

[目的]研究七叶一枝花种子萌发的最适赤霉素（GA3）浓度和组织培养的最适培养基。[方法]采用不同浓度GA3处理种子，研究促进种子快速萌发且萌发率高的GA3浓度，再将胚根3～4 cm长的种子播于土壤中，分别在光照培养箱和自然条件下生长并观察第一片叶的长势。采用不同浓度的6－BA诱导愈伤组织，研究愈伤组织诱导的适宜6－BA浓度。[结果]150 mg／L GA3处理种子的萌发率最高，种子外植体最合适的愈伤组织诱导培养基为添加0．1 mgL／6－BA和0．5 mg／L NAA的1／2MS基本培养基。[结论]七叶一枝花种子的快速萌发方法是可行的。%Objective] To research the optimal GA3 concentration and the optimal culture medium for seed germination of Paris polyphylla Smith.[ Method]Seeds of P.polyphylla were processed by different concentrations of GA3 .The optimal GA3 concentration to promote the rapid seed germination and high germination rate was researched.Seeds with 3-4 cm radicle were planted in the soil.Under the condition of illumina-tion and natural growth, growth of the first leaf was observed, respectively.Callus were induced by different concentrations of 6-BA, so as to find the optimal concentration of 6-BA.[Result] In 150 mg/L GA3 treatment, seeds had the highest germination rate.The optimal callus induction medium for seed explants was 1/2 MS +0.1 mg/L 6-BA +0.5 mg /L NAA.[Conclusion] This ratpid germination method for P.polyphylla is feasible.