治疗奶牛乳房炎的中药组方工艺研究

摘要

[Objective] To study the technology of traditional Chinese medicine in the treatment of mastitis in dairy cows.[ Method] Orthogo-nal test was conducted to screen out suitable grinding time and mixing ratio;TLC method was adopted to establish detection method for effective components in new medicines.[Result] The optimal preparation conditions were crushing time 45 min, mixing ratio 1.0:1.0:0.4;TLC iden-tification results of Taraxacum mongolicum, Rhapontisum unflorum, Angelica sinensis, Ligusticum wallichii were determined.Fluorescent spots of the same color appeared in the corresponding position of the control product chromatography.[ Conclusion] Optimal grinding time and mix-ing ratio is a safety and effective method for prescription preparation.Established test items can be used for quality control of effective constitu-ents.%[目的]研究中药治疗奶牛乳房炎的最佳制备工艺.[方法]通过设计,筛选较适宜的粉碎时间和混合比例;采用薄层色谱法(TLC)建立新中药组方有效成分的检测方法.[结果]确认了该组方最佳制备工艺为粉碎时间45 min,混合比例为1.0:1.0:0.4;确定有效成分中蒲公英、漏芦、当归、川芎薄层鉴别结果,供试品色谱中,均在与对照品色谱相应的位置上出现相同颜色的荧光斑点.[结论]优选粉碎时间和混合比例是组方制备的安全有效方法.建立的检测项可以用于有效成分的质量控制.