[Objective] To explore effects of trace metal elements on middle and low temperature anaerobic digestion process.[Method] Effects of trace elements (Fe,Co,Ni) on anaerobic digestion system were investigated through batch experiments at 20 ℃ and 35 ℃.[Result] The re-sults showed that the consuming velocity of substrate was faster by anaerobic digestion and the aerogenesis rate was higher at 35℃,while,the ac-tivity of psychrophilic methanogens was higher at 20 ℃.The pH of all treatment groups maintained from 6.5 to 7.0 which benefited the reactions of anaerobic fermentation.Especially,methane content was higher in biogas produced at 20 ℃ and relative average methane content in WP20 group reached to 52%.Meanwhile,the effects of adding trace metal elements on different strains of bacteria were also different.The effect was most significant in W group at 20℃,but in G1 group at 35℃,and the cumulative biogas yield of G1P35 group was up to 7129 mL.[Conclusion] The study can provide the technical support for the further study on the microbial characteristics,environmental factors interaction,the temporal and spatial distribution of bacteria and the population dynamics in the low temperature condition.%[目的]探究微量金属元素对中低温厌氧消化工艺的影响.[方法]通过向序批式反应体系内投放微量金属元素(Fe、Co、Ni),研究不同温度条件下,厌氧消化效率的差异.[结果]35℃条件下,厌氧发酵对底物消耗更为迅速,产气效率更高,而20℃条件下,则更有利于刺激嗜冷产甲烷菌群的活性,各处理的pH多维持在6.5~7.0,更适宜厌氧发酵反应的进行.其产生的沼气,甲烷含量也相对更高,其中WP20处理平均甲烷含量可达52%.而投放微量金属元素,对不同菌种的刺激效果存在一定差异,在20℃条件下,微量元素对W菌种厌氧发酵产气效率影响最为显著;35℃条件下,微量元素对G1菌种影响较为明显,G1P35累计产气量达7129 mL.[结论]该研究可为深入探讨低温条件下沼气生物强化菌群的微生物特性、环境因子互作、菌群时空分布和种群动力学提供技术支撑.
展开▼
