广东省禽源大肠杆菌分离株的耐药性调查

摘要

[Objective]To understand the antibiotic resistance of Escherichia coli from poultry source in different areas of Guangdong Province. [Method]Using paper diffusion method,the drug resistance of 229 isolates of E.coli from different poultry farms to 28 kinds of antimicrobial a-gents was tested.[Result]The drug resistance rates of 229 isolates of E.coli to tetracycline,compound sulfamethoxazole,amoxicillin,ampicil-lin, doxycycline and streptomycin were 89.1%,80.0%,76.9%,75.5%,74.7% and 68.6% respectively, but their drug resistance rate to amikacin was the lowest(only 8.3%).There was multi-drug resistance in 229 isolates of E.coli, among which 6 isolates were resistant to 26 drugs.[Conclusion]E.coli isolates from poultry source in Guangdong Province had severe antimicrobial resistance, and multi-drug resistance phenomena existed.It was suggested that rational use of drugs should be made in farms and the monitoring of antimicrobial resistance should be strengthened.The research provided effective theoretical basis for selecting suitable drugs,controlling the generation and diffusion of antimicrobial resistance.%[目的]了解广东省不同地区禽源大肠杆菌的耐药情况.[方法]采用纸片扩散法对不同养禽场分离的229株大肠杆菌进行28种抗菌药物的耐药性试验.[结果]229株大肠杆菌对四环素、复方磺胺甲噁唑、阿莫西林和氨苄西林的耐药率较高,分别为89.1%、80.0%、76.9%和75.5%,其次是多西环素和链霉素(74.7%和68.6%),但阿米卡星耐药率最低(8.3%).229株大肠杆菌存在多重耐药性,其中6株大肠杆菌可耐26种药物.[结论]广东省禽源大肠杆菌分离株耐药情况严重,存在多重耐药现象.养殖场应合理使用药物,加强细菌耐药性监测,研究结果为养殖场选用合适药物、控制细菌耐药性的产生和扩散提供了有效的理论依据.