一个水稻与稻瘟病菌(Magnaporthe grisea)互作相关新基因的克隆

摘要

A PCR-based differential screening method was applied to clone genes induced by Magnaporthe grisea with a pair of rice near isogenic lines(NILs)H7R and H7S. Northern blot hybridization showed that one cDNA transcript RIM9b(712 bp in length)accumulated in both resistant(H7R)and susceptible(H7S)rice lines inoculated with M.grisea.The cDNA RIM9b has a 558 bp ORF. Southern blotting hybridization with genomic DNAshowed that RIM9b is present in single copy.The results suggest that RIM9b expression might be an early rice response to infection by the pathogen.%以水稻抗瘟性近等基因系H7R和H7S为材料，采用PCR-差别筛选(PCR-baseddifferential screening)的方法分离和克隆了一个受稻瘟病菌诱导表达的基因，RIM9b。Northern杂交显示该克隆为受稻瘟病菌诱导的早期反应基因（片段），其转录丰度在诱导12 h达到最高峰。Southern 杂交结果表明该基因以单拷贝存在于水稻基因组中。序列分析表明该克隆包含一个558 bp的开放阅读框，其序列与GenBank中数据无同源性。