Banded leaf and sheath blight (BLSB) becomes an emerging worldwide problem in maize production,which caused by the fungal pathogen Rhizoctonia solani K(ǔ)hn.High-resistance lines R15 and susceptible lines Ye478 were selected as materials to elucidate the expression mechanisms of protein in maize leaves infected by R.solani Kǔhn, Total proteins were extracted from inoculated leaves during different times (0, 12, 24, 36 and 48 h) respectively and then analyzed by IEF/SDS-PAGE gel electrophoresis technique.Eight hundred protein spots were obtained, and of which, several proteins showed differences in expression levels and fifteen different protein spots were selected to analysis by ionization flight time mass spectrum facilitated by matrix auxiliary laser, and combining with the bioinformatics analysis and on-line database.It is showed that several differential proteins were induced by R.solani Kǔhn in different maize inbred lines.Of which, Shikimate kinase I , Ferredoxinthioredoxin reductase catalytic chain (FTR) increased their expressions which induced the resistance system startup, while photo-system I reaction center subunit N, Ribulose-bisphosphate arboxylase/oxygenase large subunit ruduced their expressions which ihibited the material and energy metabolism, and then made the susceptible plants death.%由立枯丝核菌(Rhizoctonia solani Ktihn)引起的纹枯病是玉米产业一个突出的世界性难题,为探讨玉米纹枯病发病后的蛋白表达机制,本研究以高耐纹枯病玉米(Zea mays)自交系R15和高感自交系掖478为材料,分时段(0、12、24、36和48 h)提取接菌后叶片总蛋白,利用IEF/SDS-PAGE凝胶电泳技术,对抗、感材料的总蛋白表达差异结果进行了比较分析.结果表明:在蛋白表达谱600～800个清晰蛋白点中,部分蛋白质(多肽)的表达量在两自交系中存在着一定的差异;对其中15个差异表达蛋白进行了基质辅助激光解析电离飞行时间质谱(MALDI-TOF/MS)分析鉴定,并且结合数据库和生物信息学软件分析,确定了莽草酸激酶Ⅰ、铁氧-硫氧蛋白还原酶催化亚基(FTR-C)等蛋白增量表达,而磷酸核酮糖羧化酶/加氧酶大亚基、光合系统Ⅰ反应中心N亚基等蛋白减量表达,其中增量表达的蛋白能诱导抗病系统的启动,而减量表达的蛋白会抑制物质和能量代谢途径,最终导致植株感病死亡.
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