Objective To explore the MMP - 2 and TIMP - 2 expression in doxorubicin nephropathy in rats and captopril on the im-pact of nephropathy rats. Method Male SD rats as the research object,through the tail vein injection doxorubicin method nephrotic syn-drome model was established,the control injection of saline solution. In experiment 7,14,28,and 42 days testing rats,24 hours urinary protein level and MMP - 2 and TIMP - 2 levels in serum and kidney tissue were determined by ELISA. And put to death in the rat,kid-ney tissues did light microscope examination,to observe changes in renal morphology of rates. 42 days with the conventional method to de-tect the serum triglyceride(TG),total cholesterol(TC),albumin(propagated),total protein(TP),creatinine(Scr),urea nitrogen ( BUN). Results ①at different times for 24 h urine protein quantitative treatment group was obviously lower,and compared with normal group and model group had significant difference( P﹤ 0. 01). ②total cholesterol,triglycerides,treatment group was obviously lower in different periods at the same time the normal group,model group( P﹤ 0. 01);Albumin,total protein was significantly higher than the lat-ter two( P﹤ 0. 01);Similar creatinine and urea nitrogen content between the 3 groups( P﹥ 0. 05). ③the application in different peri-ods after captopril treatment group serum and kidney tissue concentrations of MMP - 2 and at the same time model group than lower( P﹤0. 01),and 24 h urine protein between quantitative and were positively correlated(r = 0. 859,P﹤ 0. 01 and r = 0. 902,)and TIMP -2 with the model group than higher( P﹤ 0. 01). Conclusion MMP - 2 and TIMP - 2 with nephrotic syndrome( PNS)is closely related to the occurrence and development. Captopril has the effect of degradation of MMP - 2.%目的：探讨MMP-2和TIMP-2在多柔比星肾病大鼠中的表达及卡托普利对其影响。方法：以雄性SD大鼠为研究对象，通过尾静脉注射多柔比星的方法建立肾病综合征模型，对照组注射生理盐水。于实验第7、14、28天及第42天检测大鼠24 h尿蛋白水平，同时用酶联免疫法（ ELISA）测定血清和肾组织中MMP-2和TIMP-2水平。处死大鼠，取肾组织做光镜检查，观察各组大鼠肾脏形态学改变。第42天用常规方法检测血清三酰甘油（ TG）、总胆固醇（ TC）、白蛋白（ Alb）、总蛋白（ TP）、肌酐（ Scr）、尿素氮（ BUN）。结果：①不同时期治疗组24 h尿蛋白定量明显降低，与同时期正常组及模型组比较，差异均有统计学意义（ P﹤0.01）。②不同时期治疗组总胆固醇、三酰甘油明显低于同时期正常组、模型组，差异有统计学意义（ P﹤0.01）；白蛋白、总蛋白明显高于后两者，差异有统计学意义（ P﹤0.01）；肌酐、尿素氮含量3组间相似，差异无统计学意义（ P﹥0.05）。③应用卡托普利后不同时期治疗组血清及肾组织MMP-2的浓度与同时期模型组比降低，差异有统计学意义（ P﹤0.01），且与24 h尿蛋白定量与之间呈正相关（r=0.859，P﹤0.01；r=0.902）；而TIMP-2与模型组比则升高，差异有统计学意义（P﹤0.01）。结论：MMP-2及TIMP-2与肾病综合征（ PNS）的发生及发展密切相关。卡托普利有降解MMP-2的作用。
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