CCl4诱导大鼠肝硬化模型方法的探讨

摘要

Objective To study the method of carbon tetrachloride - induced cirrhotic model in rats. Method Forty Wistar rats were randomly divided into control group(n = 10)and model group(n = 30). Model group was given a single intraperitoneal injection with CCl4 at the concentration of 0. 2 ml/ 100 g. Then CCl4(40% )soybean oil solution was given by intraperitoneal injection twice every seven days at the concentration of 0. 3 ml/ 100 g. Control group was injected intraperitoneally with the same concentration of soybean oil solution. On the 7 weeks,Abdominal aortic blood samples were taken and the contents of SOD and MDA were determined. The livers were taken to calculate the liver coefficient,and a piece of liver was fixed in 10% formaldehyde solution to research on the tissue slices. Results There was no sig-nificant increase of body weight in model group. The liver nodules were observed and the liver coefficient was higher than control group (P ﹤ 0. 05). The formation of pseudolobuli was observed,the level of MDA was increase and the level of SOD was reduce in model group (P ﹤ 0. 05). Inthe model group,8 animals died,and modeling success rate was 73. 33%. Conclusion The speed of the method of carbon tetrachloride - induced cirrhotic model in rats is fast,modeling success rate is high.%目的：探索 CCl4诱导大鼠肝硬化模型的方法。方法：取 Wistar 大鼠40只，随机分为对照组（10只）和模型组（30只）。模型组给予0.2 ml/100 g CCl4原液腹腔注射1次，其后给予40% CCl4豆油溶液0.3 ml/100 g 腹腔注射，每周注射2次。对照组给予相同浓度的豆油溶液腹腔注射。连续注射7周后腹主动脉采血，检测 SOD 及 MDA 的含量。摘取肝脏称重，计算脏器系数，切取部分肝脏固定于10%甲醛溶液急性组织病理学观察。结果：模型组体重无明显增长，肝脏表面有结节，且脏器系数明显高于对照组（P ﹤0.05），组织切片 HE 染色后可见假小叶，血中 MDA 含量明显升高，而 SOD 活性明显降低（P ﹤0.05）。模型组动物死亡8只，造模成功率为73.33%。结论：利用 CCl4诱导大鼠肝硬化的方法速度速，模型成功率高。