紫外分光光度法测定淫羊藿颗粒中淫羊藿苷的含量

摘要

目的:建立淫羊藿颗粒中淫羊藿苷的含量测定方法.方法:采用紫外分光光度法测定淫羊藿颗粒中淫羊藿苷的含量.以甲醇作空白对照,检测波长:270 nm.结果:淫羊藿苷量在0.048 ～ 0.40 mg范围内与吸收度呈良好的线性关系,r=0.999 9,平均回收率为98.88％,RSD为2.87％.结论:本方法简便可行、结果准确可靠,重复性好,可作为淫羊藿颗粒中淫羊藿苷的含量测定方法.%Objective To establish a method for determing Icariin in Epimedium breviconum Grain. Methods UV deternina-tion of Icariin in Epimedium breviconum Grain. Using methanol as blank control, detection wavelength:270 nm. Results I-cariin in 0.048 ~ 0.400 mg Range and absorbance was linear regression, r = 0.999 9, the average recovery was 98.88 % , RSD 2.87 % . Conclusion The method is simple and reproducible for the determination of Icariin in Epimedium breviconum Grain by UV.