高效液相色谱法测定木通属植物苯乙醇苷B含量

摘要

以江西、湖南、四川、安徽4省的38份木通植物为试材，采用高效液相色谱法建立木通属植物苯乙醇苷B含量的测定方法，并测定木通藤茎中苯乙醇苷B的含量。结果表明：1)采用85%甲醇回流提取1 h，可以完整提取木通苯乙醇苷B；木通苯乙醇苷B在0.04～1μg呈良好的线性关系, R2=0.9991,平均回收率99.44%。2)木通苯乙醇苷B在三叶木通、五叶木通、白木通藤中均含有，含量0.01%～1.194%，其中在三叶木通中的成熟藤中含量最大。%The method of determination phenylethanoid glycosides B content in Akebia plant had been researched. Use HPLC to determine the content of phenylethanoid glycosides B in 38 copies of A. stem which came from Jiangxi, Hu'nan, Sichuan and Anhui Province. The results showed that: (1) Phenylethanoid glycosides B can be complete extracted by 85%methanol reflux extraction 1 h, and showed a good linear relationship in 0.04 ~ 1μ g when use HPLC, (R2=0.9991) and the average recovery was 99.44%. (2) Phenylethanoid glycosides B contained in the stem of A. trifoliata, A. quinata, A. trifoliata, fluctuated from 0.01%to 1.194%, and the content in the mature A. trifoliata was the largest.