阿魏酸钠对抗结核药物致大鼠肝损伤的保护作用及其机制研究

摘要

In order to investigate the protective effects of sodium ferulate (SF) on the hepatic injury induced by antituberculosis drugs in rats,the paper divided randomly sixty male SD rats into 6 groups:normal group,model group (isoniazid and rifampin),SF (30g/kg,60g/kg,120g/kg)and tiopronin group (60mg/kg).One hour after the final treatment,the alanine aminotransferase (ALT),aspartate aminotransferase (AST),interleukin-1β (IL-1β),IL-6, tumor necrosis factor-α (TNF-α)levels in serum were measured.Expression of TNF-α and nuclear transcription factor-κB (NF-κB)protein levels were assayed by Western Blotting method.Compared to vehicle-model group,SF at medium and high doses significantly reduced serum ALT, AST,IL-1β,IL-6 and TNF-α levels,and down-regulated liver TNF-α and NF-κB protein levels.The results showed that the protective effects of SF on the hepatic injury induced by antituberculosis drugs in rats may be related to its inhibition of NF-κB.%为探讨阿魏酸钠（SF）对抗结核药物肝损伤的保护作用机制，文章将60只SD雄性大鼠随机分为6组，即对照组、模型组（异烟肼+利福平）、SF低、中、高剂量组（30g/kg，60g/kg，120g/kg）和硫普罗宁组（60 mg/kg），给药8周。分别测定大鼠血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、白介素-1β（IL-1β）、IL-6和肿瘤坏死因子-α（TNF-α）水平。采用Western Blotting方法测定大鼠肝脏TNF-α和核转录因子-κB（NF-κB）蛋白表达水平。与模型组比较，SF中高剂量组均显著降低血清ALT，AST，IL-1β，IL-6和TNF-α水平，并降低肝脏TNF-α和NF-κB蛋白表达水平。结果证明，SF对抗结核药物肝损伤的保护作用机制可能与抑制NF-κB表达有关。