Objective To evaluate the effects of angiopoietin-1 (Ang-1) on cell apoptosis of human gastric cancer cell line BGC-823 under hypoxia. Methods BGC-823 cells were transfected with Ad-Ang-1 transiently under hypoxia induced by nimic cobalt chloride(group A). The BGC-823 cells,which were not transfected but were under hypoxia(group B),or transfected with Ad-Ang-1 without hypoxia(group C), or were not transfected(group D), were taken as the controls. The apoptosis rate was examined by flow cytometry and expressions of Bcl-2 and Bax mRNA and proteins were detected by semiquamitative RT-PCR and Western blot. Results Compared to groups of B, C and D, the apoptosis rate was lower(P＜0.01), the expression of Bcl-2 mRNA and proteins was higher, while the expression of Bax mRNA and proteins was lower in group A(P＜0. 05). Conclusion Under hypoxia,transfection of Ang-1 into human gastric cancer line BGC-823 can significantly up-regulate Bcl-2 and down-regulate Bax expressions,which may be one of the mechanisms for inhibiting apoptosis.%目的 探讨在低氧环境下将促血管生成素1(Ang-1)转染到人胃癌细胞BGC-823中,检测其对人胃癌细胞凋亡的影响.方法 利用腺病毒作为载体将已构建成功的Ang-1基因的重组腺病毒瞬时转染人胃癌细胞株BGC-823.实验分为对照组(未转染Ang-1的正常胃癌细胞)、低氧组(仅以低氧诱导剂氯化钴进行低氧干预)、转染组(仅以Ang-1转染)和低氧转染组(低氧干预加Ang-1转染).通过流式细胞术检测转染各组凋亡率的改变,再分别使用半定量RT-PCR和Westem blot 方法 检测上述各组Bcl-2和Bax mRNA和蛋白表达水平.结果 与对照组、低氧组和转染组比较,低氧转染组胃癌细胞凋亡率明显下降(P＜0.01);Bcl-2 mRNA和蛋白表达水平明显增加,Bax mRNA蛋白水平明显减少(P＜0.05).结论 低氧环境下转染Ang-1能够明显上调人胃癌细胞Bcl-2的表达,下调Bax的表达.这可能是其抑制细胞凋亡的机制之一.
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