Objective To investigate the mRNA expressions and clinical significance of cytokeratinl9(CK19) and CK20 in peripheral blood of the patients with laryngeal squamous cell carcinoma (LSCC) and hypopharyngeal squamous cell carcinoma ( HSCC). Methods The mRNA expressions of CK19 and CK20 in peripheral blood were detected by RT-PCR in 50 cases of LSCC and HSCC(group A) and 20 cases of normal controls (group C). Results The mRNA expressions of CK19 and CK20 in group A were significantly higher than those in group C(0. 4626 + 0. 122 vs. 0. 1712+0.0561 and 0.4817 + 0. 1401 vs. 0. 2175 + 0. 0704) (P<0. 01). The mRNA expressions of CK19 and CK20 during advanced stage in group A were higher than those during early stage (P<0. 05). Moreover, the expressions of CK19 and CK20 in patients with lymph node metastasis were slightly higher than those without lymph node metastasis(P＞0.05). Conclusion The detection of mRNA expressions of CK19 and CK20 in the peripheral blood is helpful for the diagnosis in LSCC and HSCC.%目的 探讨喉癌、喉咽癌患者外周血中细胞角蛋白(CK)19 mRNA、CK 20 mRNA的表达及临床意义.方法 采用实时荧光定量RT-PCR检测50例喉癌、喉咽癌患者(A组)和20例正常人群(C组)外周血CK19和CK20 mRNA的表达水平.结果 A组外周血中CK19和CK20 mRNA表达明显高于C组(0.4626±0.1220 vs.0.1712±0.0561和0.4817±0.1401 vs.0.2175±0.0704)(P＜0.01).A组晚期患者CK19和CK20 mRNA的表达高于早期患者(P＜0.05)；淋巴结转移患者外周血中CK19和CK20 mRNA表达略高于无淋巴结转移患者(P＞0.05).结论 检测外周血CK19和CK20 mRNA表达有助于喉癌、喉咽癌的诊断.
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