外源丛枝菌根真菌对低温胁迫下蓝莓幼苗抗氧化系统的影响

摘要

Four kinds of mycorrhizal fungi were inoculated in the seedlings of blueberry to investigate the coloniza-tion and the influence on low temperature-stressed in vitro branches 90 days later. All four mycorrhozal fungi colonized in blueberry in vitro branches;Coprinus sp. showed the highest colonization rate, and Tricholoma sp., the lowest. Under low temperature stress, the activities of SOD, POD and CAT were increased in mycorrhizal fungi-inoculated blueberry;the highest enzymes activities were presented in Boletus sp. treated blueberry, and the lowest, in Tricholoma sp..%本研究通过筛选得到4种菌根真菌(硬皮马勃属Scleroderma sp.、牛肝菌属Boletus sp.、鬼伞菌属Copri-nus sp.、口蘑属Tricholoma sp.),分别采用菌液浇施的方法接种蓝莓幼苗,接种90 d后进行菌根侵染率调查并进行低温胁迫处理.结果表明,4种菌根真菌均可以侵染蓝莓幼苗,其中以接种Coprinus sp.处理的侵染率最高,接种Tricholoma sp.处理的侵染率最低.2个蓝莓品种的幼苗接种不同菌根真菌后,在低温胁迫下其超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性总体高于对照,其中以接种Boletus sp.处理的蓝莓幼苗表现最好,接种Scleroderma sp.处理和接种Coprinus sp.处理也在一定程度上提高了蓝莓幼苗的酶活性,接种Tricholoma sp.处理对蓝莓幼苗酶活性的影响最小.