特应性皮炎患者外周血辅助性T细胞22的表达及意义

摘要

[Objective]To detect the expression of peripheral blood T helper cell 22 (Th22) in patients with atopic dermatitis (AD),and approach its role in AD pathogenesis.[Methods]The patients' conditions were evaluated by Eczema area and severity index (EASI) score.The peripheral blood Th22 (IL-22) cells of 46 patients with AD and 28healthy controls were detected by flow cytometry..Serum 1L-22,IFN- γ and IL-4 level were detected by ELISA assay.[Results]Th22 cells in peripheral blood of AD patients (2.35 ± 0.64)％ were significantly higher than those in the control group (0.94 ± 0.43)％ (P＜ 0.01).There was a positive correlation between Th22 cells and serum IL-22 (r=0.48,P ＜ 0.05) and no correlation between Th22 cells and IFN- γ,IL-4 (r=0.105,r=-0.267,P＞ 0.05),Th22 cells and EASI score of the disease was positively correlated (r=0.53,P ＜ 0.05).[Conclusion]Th22 cells may be used as the index of monitoring AD severity or disease fluctuations.%[目的]检测特应性皮炎(AD)患者外周血辅助性T细胞22(Th22)的表达,探讨其在AD发病机制中的作用.[方法]以湿疹面积及严重度指数(EASI)评分评估患者病情,采用流式细胞术检测46例AD患者和28例健康对照者外周血Th22(IL-22)细胞,ELISA法检测血清中IL-22、IFN-γ、IL-4水平.[结果]AD患者外周血Th22细胞(2.35±0.64)％,明显高于对照组的(0.94±0.43)％(P＜ 0.01); Th22细胞与血清中IL-22呈正相关(r=0.48,P＜0.05),而与IFN-γ、IL-4无相关(r=- 0.105,P＞0.05；r=- 0.267,P＞0.05),Th22细胞与疾病的EASI评分呈正相关(r=0.53,P＜0.05).[结论]Th22细胞有可能作为监测AD严重程度或病情波动的指标.