人类β防御素-2在舍格伦综合征患者中的表达及其意义

摘要

目的研究人类β防御素(hBD)-2及其mRNA在舍格伦综合征(SS)患者涎腺组织中的表达情况，探讨hBD-2在SS临床以及病理过程中的作用.方法采用免疫组织化学染色SP法及原位杂交(ISH)技术检测30例SS患者涎腺组织和10例正常涎腺组织中hBD-2的表达情况，并统计分析了其表达、临床病理特征之间的关系.结果 SP法和ISH的结果均提示， SS患者较正常的涎腺组织中hBD-2的表达水平增高(P<0.05)；伴有全身结缔组织疾病的SS患者较未伴有的hBD-2阳性表达率升高；病理分级4级的SS患者较3级SS患者的hBD-2阳性表达率高(P<0.05).结论 hBD-2可能参与了SS的特异性免疫病理过程，为利用唾液进行早期诊断涎腺疾病的易感性或风险因子提供了新的思路.%Objective To explore the expressions of human β-defensin(hBD)-2 and hBD-2 mRNA in salivary glands of patients with SjÖgren syndrome(SS) and to explain the correlation between hBD-2 and clinical, clinico-pathologic features. Methods To detect the expression of hBD-2 by streptaividin perodaxe immunohostochemical and in situ hybridization method from 30 cases suffered from SS and 10 cases from normal salivary glands. Corre-lations between hBD-2 and Chisholm’s score or organic manifestations were investigated. Results hBD-2 was ob-served in salivary glands from patients with SS, but few hBD-2 positive cells could be seen in salivary glands in the normal specimens. The high score of Chisholm’s score and SS with other organic manifestations were also cor-related with a higer percentage of hBD-2 of SS patients. Conclusion hBD-2 was overexpressed in glands’ ductal epithelial cells in SS and related with the chronic inflammation campared with normal specimens and may play an important role in the local autoimmune reaction.