应用ROC曲线评价P53抗体及核内小核糖体蛋白U1-A抗体对非小细胞肺癌的诊断价值

摘要

目的 评价P53抗体和U1-AsnRNP(核内小核糖体蛋白U1-A)抗体检测对非小细胞肺癌的诊断价值和临床意义.方法 采用酶联免疫吸附法检测了46例非小细胞肺癌患者血清中的P53抗体及U1-AsnRNP抗体,并以43名健康人血清作对照.结果非小细胞肺癌组血清P53抗体浓度的中位数(四分位数间距)[25.38 μg/L(65.69 μg/L)]明显高于对照组血清P53抗体浓度的中位数(四分位数间距)[10.45 μg/L(10.44 μg/L)],两组间比较差异有统计学意义(P =0.000)；非小细胞肺癌组血清U1-AsnRNP抗体浓度的中位数(四分位数间距)和对照组血清U1-AsnRNP抗体浓度的中位数(四分位数间距)分别为165.57 U/L(104.51 U/L)、170.05 U/L(74.12 U/L),两组间比较差异无统计学意义(P=0.715);应用ROC曲线对P53抗体和U1-AsnRNP抗体对非小细胞肺癌的诊断价值进行了评价,ROC曲线下面积分别0.717、0.478,P53抗体诊断非小细胞肺癌的敏感性和特异性分别为62.2％、79.1％.结论 P53抗体对非小细胞肺癌的诊断有较好的敏感性和特异性,血清P53抗体检测对非小细胞肺癌诊断有一定的临床意义；U1-AsnRNP抗体对非小细胞肺癌的诊断价值欠佳.%Objective To evaluate the diagnostic value and clinical significance of the detection of P53 antibody and small nuclear ribosomalprotein U1-A (U1-AsnRNP) antibody to non-small cell lung cancer (NSCLC).Methods Forty-six patients with NSCLC were tested for P53 antibodies and U1AsnRNP antibodies by enzyme linked immunosorbent assay,forty-three healthy persons were recruited as controls.Results The concentration of serum P53 antidbody in patients was obviously higher than that in control group [25.38 μg/L(65.69μg/L) vs 10.45 μg/L(10.44 μg/L)].There was statistical significance between the two groups ( P =0.000).The concentration of serum U1-AsnRNP antidbody in patients was 165.57 U/L ( 104.51 U/L),and it was 170.05 U/L (74.12 U/L) in control group.There was no statistical significance between the two groups ( P =0.715).ROC curve analysis was done in order to evaluate their diagnostic value to NSCLC,which showed the area under the ROC curve of P53 antibody and U1-AsnRNP antibody were 0.717 and 0.478.P53 antibody sensitivity and specificity of diagnosis to NSCLC were 62.2％ and 79.1％,respectively.Conclusions The P53 antibody for the diagnosis of NSCLC demonstrates better sensitivity and specificity,and the decection of serum P53 antibody has certain clinical significance in diagnosis of NSCLC.The U1-AsnRNP antibody has poor value in diagnosis of NSCLC.