川芎嗪对离体豚鼠气道平滑肌的效应及机制研究

摘要

Objective To observe the effect and mechanism of Ligustrazine ( LIG ) on the Airway smooth muscle( ASM) of isolated guinea pigs. Methods The normal guinea pig bronchus were made into tra-chea spiral,and randomly divided into LIG group, LIG+Propranolol group, LIG+Atropine, LIG+Nifedipine group,LIG+Glibenclamide group and the control group. The effect of different concentrations of LIG on the ASM was respectively observed in the resting tension and the high tension caused by histamine,and the ASM re-laxation rate were compared between each groups. Results (1)It had diastolic function for LIG on the isolated guinea pigs ASM in the resting tension and the high tension caused by histamine. (2)In the resting tension,com-pared with the group LIG,the diastolic function was antagonism by the concentration of LIG 10-7 ~10-6. 5 mol/L in the group LIG+Propranolo (P<0. 05). The diastolic function had no significant difference by the concentra-tion of LIG 10 -7.5 ~10 -3 mol/L in the group LIG+atropine and LIG+Glibenclamide(P>0. 05). The diastolic function was improved remarkably by the concentration of LIG 10 -6 ~10 -4 mol/L,in the group LIG+Nifedip-ine (P<0. 05). (3)In the high tension caused by histamine,compared with the group LIG,the diastolic function was antagonism obviously by the concentration of LIG 10 -6. 5 ~ 10 -5 mol/L in group LIG+Propranolol ( P<0. 05);The diastolic function had no significant difference by the concentration of LIG 10 -8 ~10 -3 mol/L in group LIG+atropine,and LIG+Glibenclamide (P>0. 05);The diastolic function was improved remarkably by the concentration of LIG 10 -6.5 ~10 -5 mol/L in group LIG+Nifedipine (P<0. 05). Conclusion It has dias-tolic function for LIG on the isolated guinea pigs ASM in the resting tension and the high tension caused by his-tamine. The effect and mechanism may be related with the competitive exciting beta 2 receptor and the inhibitor of Ca2+ internal flow. It provides a theoretical basis for the clinical application of LIG in treating asthma.%目的：探讨川芎嗪( ligustrazine,LIG)对离体豚鼠气道平滑肌( airway smooth muscle,ASM)的效应及机制。方法取正常豚鼠支气管,制作成气管螺旋条,随机分成LIG组、LIG+普萘洛尔组、LIG+阿托品组、LIG+硝苯地平组、LIG+格列苯脲组、对照组。分别观察ASM在静息张力下以及组胺所致的高张力下,各组不同LIG浓度对ASM的效应并比较各组间ASM舒张率。结果(1) LIG对离体豚鼠ASM静息张力以及组胺所致的高张力均具有舒张作用。(2)离体豚鼠ASM在静息张力下,与LIG组比较,LIG+普萘洛尔组在LIG浓度10-7~10-6．5 mol/L时,舒张作用明显受到拮抗( P <0．05)。 LIG +阿托品组、LIG+格列苯脲组在LIG浓度10-7．5~10-3 mol/L时,舒张作用没有明显差别(P>0．05)。 LIG+硝苯地平组在LIG浓度10-6~10-4 mol/L时,舒张作用明显加强( P<0．05)。(3)离体豚鼠ASM在组胺所致的高张力下,与LIG组比较,LIG+普萘洛尔组在LIG浓度10-6.5~10-5 mol/L时,舒张作用明显受到拮抗( P<0．05)；LIG+阿托品组、LIG+格列苯脲组在LIG浓度10-8~10-3 mol/L时,舒张作用没有明显差别( P>0．05)；LIG+硝苯地平组在LIG浓度10-6.5~10-5 mol/L时,舒张作用明显加强(P<0．05)。结论 LIG可对离体豚鼠ASM静息张力以及组胺所致的高张力产生明显的舒张作用,其效应的产生可能与竞争性激动β2受体以及抑制Ca2+内流有关,这为临床应用川芎嗪治疗支气管哮喘提供了理论依据。