同型半胱氨酸循环酶法自动分析仪测定及临床应用

摘要

目的 探讨同型半胱氨酸(Hcy)循环酶法在自动分析仪测定的方法评价及在脑血管疾病中的临床意义.方法 应用循环酶法测定112例高血压组、102例脑梗死组、80例健康人群血浆Hcy水平,并对结果进行分析.结果 该法Hcy在3.0～50.0 μmol/L范围内线性良好;批内CV≤3.3%,批间CV=4.6%;回收率94.4%～109.1%,平均101.2%.102例脑梗死组血Hcy水平为(14.8±5.6)μmol/L,明显高于高血压组的(11.7±5.3)μmol/L,其差异有统计学意义(P<0.01); 高血压组又高于健康对照组的(7.9±1.6)μmol/L,两者之间差异有统计学意义(P<0.01).结论 该方法简便、重复性好,适合全自动生化分析仪常规检测,Hcy水平的检测对脑血管疾病危险性的监测是1个敏感指标,对脑梗死的预后诊断具有实际应用价值.%Objective To evaluate the usage of enzymatic cycling assay in the detection of homocysteine(Hcy) and its clinical significance in the diagnosis of cerebrovascular diseases. Methods Plasma concentration of Hcy in 112 cases of patients with hyper tension,102 cases with cerebral infarction and 80 cases of healthy controls were detected by enzymatic cycling assay on automatic analyzer and the detection results were statistically analyzed. Results The linearity of this method was 3.0 50.0 μmol/L. The within run coefficien of variation(CV) was less than or equal with 3. 3％ , the between run CV was 4.6％. The range of recovery rate was 94.4 ％-109.1 ％, with an average of 101.2％. The plasma Hcy level of cerebral infarction group[ (14.8 ± 5.6)μmol/L]was significant higher than the hypertension group[ (11.7 ± 5.3)μmol/L] (P＜0.01), and that of the hypertension group was significantly higher than that of the healthy control group[(7. 9±1.6)μmol/L] (P＜0.01). Conclusion Enzymatic cycling assay is feasible, simple, with fine repeatability and suitable for routine detection on automatic analyzers. Plasma Hcy is one sensitive index for the monitoring of cerebral vascular diseases and is valuable for the prognosis of cerebral infarction.