不同方法检测乙型肝炎病毒表面抗原的结果分析

摘要

目的：分析金标免疫层析检测技术（GICA）、酶联免疫吸附测定（ELISA）和时间分辨免疫荧光分析（TRFIA）检测乙型肝炎病毒表面抗原（HBsAg）的结果。方法采用GICA、ELISA及TRFIA对11058例标本进行HBsAg检测。结果 GICA、ELISA及TRFIA法检测11058例标本HBsAg的阳性率分别为15．52％、15．64％及15．95％，两两比较，差异无统计学意义（P＞0．05）。TRFIA定量检测为0．2～＜2．0 ng/mL时，GICA、ELISA的诊断符合率分别为25．0％、75．0％。结论检测 HBsAg低浓度样本时，GICA和ELISA的诊断符合率降低。%Objective To analyze the results of gold labeled immunochromatographic assay (GICA ) ,enzyme-linked immunosor-bent assay (ELISA ) and time resolved fluoroimmunoassay (TRFIA ) detection for hepatitis B virus surface antigen (HBsAg ) . Methods GICA ,ELISA and TRFIA were employed to conduct the HBsAg testing in 11 058 specimens .Results The HBsAg posi-tive rates of GICA ,ELISA and TRFIA detection in 11 ,058 specimens were 15 .52% ,15 .64% and 15 .95% ,respectively ,with no statistically significant difference when pairwise comparison performed (P>0 .05) .In specimens which results of TRFIA quantita-tive detection was 0 .2- <2 .0 ng/mL ,the diagnostic compliance rates of GICA ,ELISA were 25 .0% and 75 .0% ,respectively .Con-clusion The diagnostic compliance rates of GICA ,ELISA detection in specimens with low concentration HBsAg are deceased .