铜绿假单胞菌的耐药性分析及Ⅰ类整合子和插入序列共同区调查

摘要

Objective To investigate antibiotic resistance of Pseudomonas aeruginosa isolated from the patients'specimens in the hospital ,the carrying rate of integronⅠ and insertion sequences common area(ISCR)and provide reference for clinical treatment . Methods 106 isolates of Pseudomonas aeruginosa were collected and their bacterial antibiotic resistance and clinical distribution were analyzed by using WHONET5 .6 software .PCR and electrophoresis were used to screen integronⅠ and ISCR carried by the target strains .Results Clinical isolates of Pseudomonas aeruginosa mainly collected from department of pulmonary disease which accounted for 48 .11% ,and sputum specimens were the major source of the 106 isolates ,which accounted for 73 .58% .The sensitiv‐ities of Pseudomonas aeruginosa to tobramycin ,amikacin ,gentamicin were about 85% ,while to imipenem only 65 .09% .The carry‐ing rate of integronⅠ and ISCR in Pseudomonas aeruginosa was 84 .91% and 76 .42% ,respectively .Conclusion Pseudomonas aeruginosa distribution of hospital departments mainly concentrate in pulmonary department and the major type of specimen is spu‐tum .The sensitivity of Pseudomonas aeruginosa to aminoglycoside antibiotic is relatively good .IntegronⅠand ISCR in Pseudomonas aeruginosa could be associated with antibiotic resistance .%目的：了解临床分离的铜绿假单胞菌的耐药性及其Ⅰ类整合子和插入序列共同区（ISCR）携带率，为临床治疗提供参考依据。方法收集从临床患者标本分离得到的106株铜绿假单胞菌，使用WHONET5．6软件分析其临床分布和耐药性，采用PCR和电泳技术对其进行Ⅰ类整合子和ISCR的检测。结果临床分离的铜绿假单胞菌科室来源以肺病科为主，占48．11％；标本来源以痰液为主，占73．58％。铜绿假单胞菌对氨基糖苷类抗菌药物的敏感率为85％左右，对亚胺培南的敏感率为65．09％，Ⅰ类整合子和ISCR的携带率分别为84．91％和76．42％。结论该院铜绿假单胞菌的科室来源以肺病科为主，标本来源以痰液为主，对氨基糖苷类抗菌药物的敏感性相对较好，Ⅰ类整合子和ISCR可能与铜绿假单胞菌的耐药性产生有关。