海藻多糖对衰老小鼠的免疫调节作用∗

摘要

目的：探讨海藻多糖(PSS)对 D-半乳糖(D-gal)所致衰老小鼠的免疫调节作用。方法腹腔注射 D-gal 制备衰老小鼠模型，同时对衰老小鼠行 PSS 灌胃，收集腹腔巨噬细胞，检测巨噬细胞分泌一氧化氮(NO)、一氧化氮合酶(NOS)的水平和诱导型一氧化氮合酶(iNOS)mRNA 表达水平；计算脾脏指数，观察 PSS 对小鼠脾脏显微结构的影响；流式细胞术检测衰老模型小鼠脾细胞周期的变化。结果PSS 能促进衰老小鼠巨噬细胞合成 NO、NOS 和上调 iNOS mRNA 表达水平，并使衰老小鼠脾脏指数明显增加，脾脏被膜层明显增生。PSS 能增加衰老小鼠脾 S 期和 G2/M 期细胞。结论PSS 能提高 D-gal 所致衰老小鼠的的免疫功能，值得进一步研究和开发。%Objective To explore the immunomodulatory effects of seaweed polysaccharide(PSS)in aged mice induced by D-ga-lactose (D-gal).Methods D-gal was injected intraperitoneally to establish the aged mice model,meanwhile the aged mice was intra-gastricly administrated by PSS.Peritoneal macrophages were collected,and macrophage secretion of nitric oxide (NO),nitric oxide synthase (NOS)levels and expression levels of inducible nitric oxide synthase (iNOS)mRNA were detected.The spleen indexs of the agd mice were calculated,and effects of PSS on spleen microscopic structure of mouse were observed.The changes of spleen cell cycle in aged model mice were detected by flow cytometry assay.Results PSS could enhance macrophage synthesis of NO and NOS of the aged mice and up-regulate the expression of iNOS mRNA levels.And the spleen index of the aged mice increased obviously, the hyperplasia of spleen capsule was obvious.Moreover,PSS could increase the percentage of S phase and G2/M phase cells of the aged mice spleen.Conclusion PSS could enhance the immune function of aged mice induced by D-gal,which is worthy of further study,which development.