中国西北地区健康人群血清总胆红素参考区间的调查

摘要

0bjective To investigate the serum total bilirubin level of healthy population in northwest area of China.Methods According to the guiding principle and the screening standard of C28-P3,722 individuals conforming to the screening standard from October 2011 to January 2012 were collected.Serum total bilirubin was determined with diazo reagent method(Roche)on the P module of Hitachi 7600 automatic biochemistry analyzer,and the vanadate reagent method(WAKO)on the D module of the Hitachi 7600 automatic biochemistry analyzer,respectively.The detection results were analyzed between different analysis systems,between country and city and among different age groups by SPSS13.0.The top and bottom limitations of 95% reference interval recommen-ded by the C28-P3 file were calculated by the non-parametric method.Results Serum total bilirubin detection results had no statis-tically significant differences between the two kinds of detection methods,between rural and urban populations,among all ages and genders (P >0.05).The ultimate reference interval of serum total bilirubin in the northwest area was 2.19 -29.29 μmol/L.Con-clusion The differences of serum total bilirubin reference interval exist between the healthy population in the Northwest area of China and the current reference interval used in domestic and foreign population.Establishing the reference intervals of new bio-chemical test item suitable for China′s population will provide the scientific basis for the evaluation of disease diagnosis,treatment, prognosis judgment and health assessment in the Chinese population.%目的：探讨中国西北地区健康人群的血清总胆红素（TBIL）水平。方法该次研究从2011年10月至2012年1月，依据 C28-P3的指导原则和筛选标准，收取符合筛选标准的人群722人，分别采用7600全自动生化分析仪 P 模块和 Roche 重氮法试剂、7600全自动生化分析仪 D 模块和日本和光 WAKO 钒酸盐试剂进行检测。用 SPSS13．0软件对不同系统间、农村与城市人群间和各年龄段间检测结果进行分析，按 C28-P3文件推荐的非参数法计算95％参考区间的上下限。结果血清总胆红素检测结果两种方法、农村与城市人群、各年龄段和性别之间均无统计学意义（P ＞0．05），西北地区血清 TBIL 最终参考区间为2．19～29．29μmol／L。结论中国西北地区健康人群血清 TBIL 参考区间与国内目前所用的及国外人群的参考区间存在差异。新的适合中国人群的生化检验项目参考区间的建立，将为中国人群疾病诊断、治疗、预后判断和健康评估提供科学依据。