目的 筛选可用于裂解多耐药鲍曼不动杆菌的噬菌体,为噬菌体治疗多耐药鲍曼不动杆菌治疗提供新的方案.方法 以上海各家医院临床分离到的多耐药鲍曼不动杆菌为宿主菌,从不同环境中分离鲍曼不动杆菌噬菌体,负染法电镜观察其形态及大小,并分析pH和温度对其稳定性的影响,并对噬菌体DNA进行测序及相关基因功能分析.结果 从医院下水道污水中分离得到了一株耐药性鲍曼不动杆菌的烈性噬菌体D218,电镜观察其为长尾噬菌体,头部呈多面体立体对称结构,直径约65 nm,尾长约100 nm,尾宽约15 nm.此株噬菌体在pH 3~11及4~50 ℃的环境下具有较高活性.其基因组全长102 449 bp,GC含量为37.2%,编码167个ORFs.结论 筛选到了一株新的对pH值及温度均稳定的耐药鲍曼不动杆菌烈性噬菌体D218,为噬菌体用于耐药性鲍曼不动杆菌的院内感染的预防控制及噬菌体库的建立增添了新的数据.%Objective To screen the bacteriophage for splitting multiple drug resistance Acinetobacter baumanii to provide a new scheme for the phage therapy of multiple drug resistance Acinetobacter baumanii infection.Methods Multiple drug resistance Acinetobacter baumanii isolated from hospitals in Shanghai served as the host bacteria.The bacteriophages were isolated from different environments.The morphology and size of bacteriophages were observed by electron microscope with negative staining.The influence of pH and temperature on its stability was analyzed.The phage DNA was extracted and sequenced.The related gene function was analyzedResults A virulent phage named D218 of drug-resistance Acinetobacter baumanii was isolated from the effluent sewerage of hospital.The electron microscopy observation showed that it is a long-tail phage of polyhedron symmetric structure,with a diameter of about 65nm,the tail length of about 100nm and width of about 15 nm.The phage kept high activity at pH 3-11 and temperature 4-50 ℃.The genome size is 102449bp with GC content of 37.2%.It encodes 167 ORFs.Conclusion A new virulent phage D218 of drug-resistance Acinetobacter baumanii is isolated,which is stable to pH and temperature,which increases the new data for its application in hospital infection prevention and control,and the phage library setup.
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