Objective To analyze the distribution and drug resistance of Streptococcus pneumoniae in 0-14 year old children of 0 to 4 year-old in theour hospital.Methods S.pneumoniae were identified by using ma-trix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).Drug suscepti-bility test was performed by using ATB STREP 5 for each isolate.Penicillin G minimum inhibitory concentra-tion (MIC) was performed by using E-test.Results A total of 327 strains of S.pneumoniae were isolated,a-mong which 287(87.8%)were from sputum,25(7.6%) from the ear secretions,10(2.9%) from the nasal sinuses puncture fluid,and 3(0.9%)from the blood.The most strains were isolated in spring(28.4%),and the least in winter (22%),and there was no significant difference in the separation rate among the four sea-sons (P<0.05).The sensitivity rate,intermediate rate and drug resistant rate of non meningitis S.pneumoni-ae strains to penicillin G were 97.9%,0.6%,1.5%,respectively.The resistance rates of the bacteria to eryth-romycin,clindamycin,compoundtrimethoprim sulfamethoxazole and tetracycline were relatively high,a small number of levofloxacin resistant strains were found,and vancomycin and quinopin /Dafoe-P resistant strains were not detected.The resistance rates of penicillin G,penicillin resistant Streptococcus pneumoniae (PRSP) and penicillin inter mediated Streptococcus pneumoniae (PISP)to amoxicillin and cefotaximeto were markedly higher than that of penicillin G sensitive Streptococcus pneumoniae (PSSP),and the difference was statistically significant (P<0.01).A total of 227 strains of multidrug resistance Streptococcus pneumoniae (69.4%)were isolated,which were resistant to erythromycin,tetracycline,trimethopri compoundm-sulfamethoxazol and clin-damycin(46.2%).Conclusion The drug resistance monitoring of Streptococcus pneumoniae in children can not be ignored.The drug resistance of the bacteria to penicillin G is low.Penicillin G is still a cheap and effec-tive drug for the treatment of Streptococcus pneumoniae infection.%目的 分析该院0~14岁儿童患者肺炎链球菌感染分布及耐药性特征.方法 使用基质辅助激光解析飞行时间质谱仪(MALDI-TOF MS)鉴定细菌,使用ATB STREP 5进行药敏试验,青霉素G最低抑菌浓度(M IC)采用 E试验法检测.结果 共分离肺炎链球菌327株,其中287株(87.8%)来自痰液,25株(7.6%)来自耳分泌物,10株(2.9%)来自鼻窦穿刺液,3株(0.9%)来自血液.菌株分离的季节以春季最多(28.4%),冬季最少(22.0%),但四季分离率差异无统计学意义(P<0.05).肺炎链球菌非脑膜炎株对青霉素G的敏感、中介和耐药率分别97.9%、0.6%、1.5%.该菌对红霉素、克林霉素、复方磺胺甲噁唑、四环素耐药率较高,已出现少量左氧氟沙星耐药株,未检出万古霉素和奎奴普丁/达福普汀耐药株.青霉素G耐药、青霉素肺炎链球菌(PRSP)、青霉素中介肺炎链球菌(PISP)对阿莫西林、头孢噻肟的耐药率显著高于青霉素G敏感的肺炎链球菌(PSSP),差异有统计学意义(P<0.01).共分离出多重耐药肺炎链球菌227株(69.4%),以红霉素、四环素、复方磺胺甲噁唑和克林霉素耐药为主(46.2%).结论 儿童患者感染肺炎链球菌的耐药性监测不容忽视,该菌对青霉素G的耐药性较低,青霉素G仍是治疗肺炎链球菌感染价廉而有效的药物.
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