目的:观察缝隙连接阻滞剂Heptanol对离体SD大鼠心脏机械功能及缺血心肌组织的影响. 方法:(1)建立离体SD大鼠心脏Langendorff灌流模型,灌流含有Heptanol的K-H灌流液,观察离体SD大鼠心脏收缩及舒张功能.(2)结扎左前降支,建立局部心肌缺血模型,观察Heptanol对缺血心脏的机械功能的作用.(3)透射电镜观察Heptanol对缺血心肌结构的影响. 结果:(1) Heptanol可以降低正常心脏的左室压力最大上升速率(dp/dt max)和左室收缩峰值(LVSP),对左室舒张末压(LVEDP)没有明显影响.(2)缺血心脏的LVSP、dp/dt max下降,LVEDP上升.Heptanol对缺血心脏的LVSP、dp/dtmax、LVEDP均无明显影响.(3)透射电镜观察可见缺血使心肌组织遭到破坏,Heptanol可以减轻组织损伤. 结论:(1)Heptanol可以使正常心脏的收缩功能下降,对舒张功能无明显影响,不会使缺血心脏的机械功能进一步下降.(2)Heptanol可以减轻由于缺血造成的心肌组织破坏.%Objective; To observe the effects of gap junction inhibitor Heptanol on hemodynamics and myocardium in isolated SD rat hearts. Methods: (1 ) The Langendorff perfusion model was established. The SD rat heart was perfused with Heptanol. The effects of Heptanol on the systolic and diastolic function were observed. (2) The regional ischemic model was established by ligation of the left anterior descending branch of coronary artery. The effects of Heptanol on hemodynarnics of isolated SD rat hearts were observed. (3) The influence of ischemia and Heptanol on myocardium was observed with transmission electron microscopy. Results: (1) Heptanol could decrease dp/dt max and LVSP, but had no effect on LVEDP in normal heart. (2) Ischemia could decrease LVSP and dp/dt max and increase LVEDP. Heptanol had no more influence on the downregulation of the heart function. (3) Transmission electron microscopy revealed that Heptanol could decrease the damage of myocardium induced by ischemia. Conclusion: (1) Heptanol could decrease systolic function of the heart, but had no effect on diastolic [unction. (2) Heptanol could reduce the damage of myocardium induced by ischemia.
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