治疗用卡介苗抑瘤小鼠模型的建立

摘要

Objective To establish a mouse model for studying tumor suppression of therapeutic BCG (tBCG).Methods tBCG treatment,positive control and negative control solutions were prepared by mixing tBCG (20,10 and 5 mg/ml),cyclophosphamide (7.2 mg/ml)and sterile sodium chloride solutions with mouse liver cancer H22 cell solution (1 × 107/ml),respectively,and BALB/c mice were inoculated subcutaneously with a kind of mixed preparations.Tumor sizes and weights of mice were measured,and average weights and suppression rates of tumor were calculated.The statistical analysis of the results was made by t tset.Results Tumor weights and sizes in tBCG treatment group( 10 mg/ml) reduced significantly as compared with those in negative group ( t =3.41,P ＜ 0.05 ; t =4.88,P ＜ 0.05 ).The results suggested tBCG treatment could suppress tumor growth.Conclusion The established mouse model for tumor suppression of tBCG can be used for studying antitumor effect of tBCG.%目的 建立治疗用卡介苗抑瘤小鼠模型,以研究治疗用卡介苗的抗肿瘤疗效.方法 分别将适宜浓度的治疗用卡介苗溶液(20、10和5 mg/ml)、环磷酰胺溶液(7.2 mg/ml)和灭菌生理氯化钠溶液与适宜浓度的小鼠肝癌H22细胞(1×107个/ml)混合制成治疗用卡介苗处理液、阳性对照液和阴性对照液,皮下接种BALB/c小鼠,接种后4周,测定小鼠的肿瘤大小和重量,计算每组小鼠的平均肿瘤重量和瘤重抑制率,采用t检验对测定的结果进行统计学分析.结果 与阴性对照组相比,10 mg/ml治疗用卡介苗处理组的肿瘤重量明显减轻(t =3.41,P＜0.05),肿瘤体积明显缩小(t=4.88,P＜0.05),表明采用治疗用卡介苗处理可使肿瘤生长受到抑制.结论 建立的治疗用卡介苗抑瘤小鼠模型可用于卡介苗的抗肿瘤疗效研究.