天然类黄酮分子中羟基基团在兔眼缺血损伤后眼球血流量和视网膜功能恢复的作用

摘要

AIM: To determine the effect of hydroxy (OH) group on the ocular blood flow and retinal function recovery.METHODS: Colored microsphere technique was used to determine the ocular blood flow in rabbit eyes and electroretinography was used to measure the retinal function recovery.RESULTS: Flavones with four free OH groups seemed to produce the optimal effects in ocular blood flow increase and retinal function recovery. When there were 3OH groups (Apigenin), rapid increment in ocular blood flow and retinal function recovery was found. When the number of OH groups was below two (7-Hydroxyfiavone,Chrysin), no effect was observed on the ocular blood flow. The attachment of rutinose group in the fiavone (Diosmin) with two free OH groups and methoxy group did not affect the ocular blood flow or retinal function recovery, but the attachment of glucose group in the fiavone (Luteolin-7-glycoside) with catechol group affected the ocular blood flow one way or the other. The attachment of methoxy group in the fiavone (Acacetin)with two free OH groups affected ocular blood flow and retinal function recovery after ischemic insult.CONCLUSION: Ocular blood flow and retinal function recovery are increased significantly with the increase in the number of OH groups attached in the flavone molecule, with the 7-OH group and the catechol group in the B ring the most efficient to enhance the ocular blood flow and retinal function recovery after ischemic insult.%目的:我们最近已报告有些天然类黄酮可影响眼球血流量.为阐明黄酮分子中羟基(OH)的作用,选择黄酮含1-4个羟基基团、附有或无糖、有或无甲氧基团,观察对眼球血流量和视网膜功能恢复的作用.方法:采用彩色微珠技术测定兔眼球血流量,视网膜电图监测视网膜功能的恢复.结果:黄酮含4个游离的羟基基团(毛地黄黄酮),似乎有增加眼球血流量和促进视网膜功能恢复的作用.含3个羟基基团(5,7,4′-三羟基黄酮)可迅速增加眼球血流量和恢复视网膜功能.少于2个羟基基团的黄酮(Chrysin),对眼球血流量无增加作用.黄酮附着有芦丁糖,并含2个游离的羟基和甲氧基团(Diosmin),对眼球血流量和视网膜功能均无作用,但是,含儿茶酚的黄酮附着有葡萄糖时(毛地黄黄酮-7-糖苷),会影响眼球血流量.黄酮附着有甲氧基,并含2个游离羟基基团(Acacetin),均影响缺血损伤后眼球血流量和视网膜功能的恢复.结论:黄酮分子中羟基基团数量的增加,可显著提高眼球血流量和促进视网膜功能的恢复,而在黄酮分子B环上有7个羟基基团和儿茶酚基团时,其作用最显著.