白蒺藜皂苷对慢性高眼压兔视网膜SOD活性和MDA含量的影响

摘要

AIM: To observe influence of gross saponins from tribulus terrestris L (GSTT) on SOD activity and MDA content for chronic high intraocular pressure in rabbit, and discusses the retina oxidative damage inhibition on chronic high intraocular pressure model of rabbit.rn METHODS: Totally 24 healthy New Zealand rabbits were randomly divided into 4 groups; normal control group (A group); high intraocular pressure model blank group (B group); high intraocular pressure model with GSTT treated group (C group); high intraocular pressure model with Erigeron brevicapas hand mass ( EBHM) treated group (D group). High intraocular pressure model was induced by 20g/L methylcellulose injection into the anterior chamber in B group, C group and D group. D group was injected 5 mg/kg GSTT and C group was injected 4. 5mg/kg EBHM and measured intraocular pressure with Schiotz tonometer every day for 4 weeks. The retina tissue superoxide dismutase (SOD) and maleic dialdehyde(MDA) content were detected 28 days later.rn RESULTS: After glaucoma model of rabbit eyes were established, the intraocular pressure during observation period was maintained in 32-39mmHg; High intraocular pressure model blank group and normal control group,rnEBHM treatment group, GSTT treatment group were compared, the differences of retina MDA, SOD content had statistical significance (P0. 05); EBHM treatment group, GSTT te atment group and normal o ntrol group were compared, the content of MDA in the retina was still slightly higher (P0.05),EBHM治疗组、GSTT组与正常对照组比较视网膜中MDA的含量仍略高(P<0.05),SOD的含量略降低(P<0.05).结论:白蒺藜皂苷能有效提高慢性高眼压下兔视网膜中SOD活性,降低MDA含量,从而对持续性高眼压视网膜氧化应激具有保护作用.