化瘀温胆汤对代谢综合征大鼠胰岛素抵抗干预作用的实验研究

摘要

目的:探讨化瘀温胆汤对代谢综合征大鼠胰岛素抵抗的治疗作用.方法:健康雄性SD大鼠高温高湿环境下喂饲高脂高糖高盐饮食12w,复制大鼠代谢综合征模型.通过检测肥胖相关指标(体重、腹围、体长,计算Lee's指数)、胰岛素抵抗指标[空腹血糖(FPG)、血清胰岛素(Fins),并计算胰岛素敏感指数(IAI)、胰岛素抵抗指数(IRI)以及胰岛素分泌指数(IS)],并以盐酸二甲双胍为阳性对照药,探讨化瘀温胆汤灌胃给药4w后对模型大鼠胰岛素抵抗的治疗作用.结果:化瘀温胆汤可明显降低模型大鼠的体重、腹围以及Lee's指数,与模型对照组比较,P<0.01;给药前后体长变化不明显.化瘀温胆汤给药后FPG、Fins、IRI较模型对照组显著减低,均P<0.01;IAI较模型对照组显著升高,P<0.01;IS变化不显著.结论:化瘀温胆汤具有明显改善代谢综合征模型大鼠肥胖指标以及改善胰岛素抵抗作用.%Objective: To observe the therapeutic effects of HuaYu Wendan Tang on insulin resistance of laboratory metabolic syndrome (MS) mice and its mechanism, and thus provide foundation for HuaYu Wendan Tang on treating MS with Traditional Chinese Medicine and Materia medica. Methods: To render mice by feeding high -lipid, high -glucose, high -salt diet in the high temperature and high humid environment and then treat them with HuaYu Wendan Tang, compared with pioglitazone. Then we observed the effects of the drugs on weight, glycol metabolism and insulin sensitivity. Results: Huayu Wendan Tang can lose mice weight, reduce abdominal circumference and index of Lee's. Serum tests of glycometabolism showed that Huayu Wendan Tang can significantly lower fasting blood sugar and insulin and thus improve IR condition, similar to the effect of mefformin. Conclusion: Huayu Wendan Tang can make mice lose weight, improve their insulin resistance.Huayu Wendan Tang may lower fasting blood sugar and insulin and thus improve IR condition of MS by minimizing insulin resistance and improving insulin sensitivity.