Objective: To investigate the effect of Tangshenkang produced on DN by observing the expression of AGES and TGF - β1 of renal tissue in STZ - induced Diabetic Nephropathy Rats. Methods:The rat models were induced by right nephrectomy and peritoneal injection of some dosage streptozotocin(50mg/kg). Ninety male Wistar rats were randomly divided into six groups: normal group, model group, Yina Puli group, Tangshenkang high - dose, middle - dose ,low - dose treatment group, and each group with ten rats. After 12 weeks of treatment , test the blood and urinary guidelines and finish the nephrectomy. The expressions of AGES and TGF - ?1 in the kidney were detected by immunohistochemistry. Results:The AGES and TGF - β1 expressed highly in the kidneys of the model rats, ECM accumulated when it was observed with microscope, while lower in the kidneys which were treated by Tangshenkang, and expressed lowest in the high - dose group, but there was no significant difference from middle -dose group. Conclusion:The Tangshenkang formula can decrease the accumulation of ECM and the expression of AGES and TGF - β1 in the kidney of DN rats , and it may be the mechanism of Tangshenkang on reducing ECM accumulation and improving the DN kidney pathology.%目的:通过观察糖肾康饮对实验性糖尿病肾病大鼠肾组织糖基化终产物(AGES)、转移生长因子β1(TGF-β1)的表达沉积,肾功能及肾脏结构的影响,进一步明确糖肾康饮治疗糖尿病肾病的作用机制.方法:切除大鼠左侧肾脏联合腹腔注射链脲佐菌素(50mg/kg)建立糖尿病肾病(DN)模型,90只雄性Wistar大鼠随机分为空白组、模型组、西药马来酸依那普利组、中药糖肾康饮高,中,低剂量组,给药12周后测血尿指标,取右侧肾脏,采用免疫组化法检测肾组织AGES和TGF-β1的表达.结果:模型组大鼠肾组织AGES和TGF-β1大量表达,镜下特征表现为肾小球细胞外基质(ECM)积聚所致肾小球基底膜、系膜增生,经12周的糖肾康饮治疗后肾组织中AGES和TGF-β1明显少于模型组,以糖肾康饮高剂量组效果最好,但与中剂量组相比无显著差异.结论:糖肾康饮能够减少ECM积聚,降低DN大鼠肾组织AGES和TGF-β1的表达,可能是通过降低AGES和TGF-β1的表达,减少肾小球细胞外基质(ECM)积聚而改善DN肾脏病理结构.
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