人参皂苷Rd抑制人脑胶质瘤U251细胞增殖机制研究

摘要

Objective:To investigate the inhibitory effects of Ginsenoside Rd on the proliferation of U251 cells in vitro.Methods:The proliferation activity was detected by MTT assay.Cell apoptosis and the change in the cycle of U251 were detected by flow cytometry.Real-time quantitative PCR was used to measure the expres-sion of Bcl-2 and caspase-3 in different groups.Results: The proliferation of U251 cells was inhibited by Ginsenoside Rd with the concentration of 20~80μmol/L to 80 μmol/L in dose dependent manner.All dose groups of Ginsenoside Rd could induce apoptosis of U251 cell.Cell cycle analysis showed that the increase of Ginsenoside Rd concentration and the proportion of cells in G1/G0 phase were increased, while that of cells in Sand G2/M phases was decreased.Compared with the blank control group, all dose groups of Ginsenoside Rd could significantly enhance the gene expression of Caspase-3 mRNA ( P<0 .05 ) , and could decrease the gene expression of Bcl-2 mRNA(P<0.05).Conclusion: The Ginsenoside Rd can remarkably restrain the multiplication of U251 glioma and promote the cell to apoptosis, and antitumor mechanisms can be related to down-regulating the expression of Bcl-2 mRNA and up-regulating the expression of Caspase-3 mRNA.%目的:体外研究人参皂苷Rd(GS-Rd)抑制人脑胶质瘤U251细胞增殖机制.方法:采用MTT法检测GS-Rd对U251细胞增殖情况的影响;采用流式细胞仪观察GS-Rd对U251细胞周期及凋亡影响;通过实时荧光定量PCR检测GS-Rd对U251细胞相关凋亡基因表达的影响.结果:1)GS-Rd在浓度为20~80μmol/L范围内对U251细胞均有一定的抑制作用,并且抑制强度随药物浓度的增加而增大,呈剂量依赖性;2)GS-Rd各剂量组均可诱导U251细胞凋亡.细胞周期分析显示随着给药浓度增加,越多的U251细胞被阻滞在G1/G0期,使进入S和G2/M期的细胞减少;3)与空白对照组相比,GS-Rd各剂量组Bcl-2mRNA表达显著降低(P<0.05),同时Caspase-3mRNA表达显著提高(P<0.05).结论:人参皂苷Rd能抑制人脑胶质瘤U251细胞增殖,同时能诱导细胞凋亡,其抑瘤机制可能与下调Bcl-2mRNA表达和上调Caspase-3mRNA表达有关.