龙牡桂枝汤对ADHD模型大鼠左右额叶-纹状体NET mRNA表达的影响

摘要

目的:观察龙牡桂枝汤对注意缺陷多动障碍(ADHD)模型动物-幼年自发性高血压大鼠(SHR)左右额叶-纹状体去甲肾上腺素转运体(NET)mRNA表达的影响,探讨该药治疗ADHD的作用机制.方法:幼年雄性SHR大鼠随机分为龙牡桂枝汤高、中剂量组、盐酸哌甲酯对照组和生理盐水对照组,连续灌胃给药14天,采用Real-time PCR法检测各组大鼠左右脑前额叶皮质、纹状体NET mRNA表达水平.结果:在右前额叶皮质,龙牡桂枝汤高剂量组NET mRNA表达较其他实验组明显增加,而在左前额叶皮质各组差异不明显.在双侧纹状体,与生理盐水组相比,各用药组NET mRNA的表达显著增加,且龙牡桂枝汤高剂量组>中剂量组>盐酸哌甲酯组,差异具有统计学意义.结论:龙牡桂枝汤治疗ADHD的可能机制之一与其上调右脑前额叶皮质及双侧纹状体NET mRNA表达有关.%Objective:To study the effect of Longmu Guizhi decoction ( LD) on the NET mRNA expression in the left and right brain of juvenile SHR ( spontaneously hypertensive rats ,rat models with attention deficit hyperac-tivity disorder(ADHD),and to investigate the mechanism of LD for ADHD .Methods:Male SHRs were ran-domly divided into a high -dose LD group ,a middle-dose LD group ,a methylphenidate control group and a saline control group .All the SHRs were lavaged for 14 days.The NET mRNA expressions in the left and right prefrontal cortex and corpus striatum were detected by the method of Real -time PCR.Results:In the right prefrontal cortex , NET mRNA expression was significantly increased in the high -dose LD group compared with the saline control group ,but there was no obvious difference in the left prefrontal cortex among all groups . In the bilateral corpus striatum, compared with the saline control group ,NET mRNA expression was signifi-cantly increased in the methylphenidate control group and two LD groups ,and the NET mRNA expressions of high-dose LD group were higher than those of the middle -dose LD group , which were higher than those of the methylphenidate control group , and the difference was statistically significant .Conclusion:The potential mechanism of LD in the treatment of ADHD is related to that it can increase NET mRNA expression in the right prefrontal cortex and bilateral corpus striatum .