Pseudomonas sp.HS-2降解双酚F及脱氮的特性研究

摘要

Pseudomonas sp. HS-2 can grow by using bisphenol Fas sole carbon source and NH4+-N as sole nitrogen source,and denitrify via the process of heterotrophic nitrification-aerobic denitrification (HN-AD). Bisphenol Fde-gradation and denitrification capabilities of strain HS-2 have been investigated under the following conditions:35℃, 150 r/min and pH 7.0. The results show that strain HS-2 could efficiently degrade 500 mg/L of bisphenol F. Under the optimal C/N ratio of 20,the removing rates of bisphenol F and NH4+-N can reach 99％and 96％in 48 h,respec-tively. In addition,it is found that during this process,ammonia monooxygenase has participated in BPF degradation. Nitrogen balance analysis demonstrates that 47.7％of initial NH4+-N is converted into N2. The additional sodium cit-rate and NO3--N have remarkable facilitating effects on the removal of bisphenol F and NH4+-N.%Pseudomonas sp.HS-2能以双酚F为唯一碳源、NH4+-N为唯一氮源进行生长,并通过异养硝化好氧反硝化过程脱氮.在35℃,150 r/min和pH=7.0条件下,考察了菌株HS-2降解双酚F的能力及其脱氮性能.结果表明,菌株HS-2能够降解500 mg/L双酚F.并且在最适碳氮比为20的条件下,双酚F和NH4+-N的去除率可分别达到99％和96％.并且发现氨单加氧酶参与了双酚F的降解.氮平衡分析表明,47.7％的初始NH4+-N转化为N2.外加柠檬酸钠或硝态氮对双酚F和NH4+-N的去除有明显的促进作用.