培养条件对赭曲霉菌球形成和坎利酮11α羟基化的影响

摘要

菌丝粗壮、分布均匀的菌球可提高赭曲霉11α羟基化的效率.控制赭曲霉菌球直径的平均大小可以通过选择不同的培养温度、装液量、以及转速等因素而达到.通过正交分析,确定培养温度为30 ℃,按孢子悬液浓度为108个/mL接种,装液量为50 mL/250 mL三角瓶,摇床转速为200 r/min时,菌球达到最佳直径2～3 mm之间的百分比最大.在此条件下,以10 g/L的坎利酮投料,最终转化率高达91.26%.%The efficiency of 11α hydroxylation could be in proved by A spergillus ochraceu pellets w ith thick hyphae and uniform distribution. T he experim ent indicated that the m ean size of pellets could be controlled by different culture temperature, inocuktion volum e, rotation speed and other factors. Through the orthogonal analysis, when the culture tem perature w as 30 ℃ , the concentration of spore suspension w as inoculated w ith 10 spores/m L , m edium volum e 50 m L/250 m L flask, shaker speed 200 r/m in, the m axin urn rate of the pellet diam eter from 2mm to 3 m m was obtained. U nder these conditions by adding 10 g/L of canrenone, the final conversion rate reached as high as 91.26% .