黑曲霉β-葡萄糖苷酶产酶条件的优化

摘要

The medium composition and culture conditions for the production of β-glucosidase by Aspergillus niger Glu05 were optimized as followers: wheat bran 4% , tryptone 4 %, 1 μmol MnSO4, 1 μmol Nad, KH2 PO4 0.2% , natural pH, rotation speed 250 r/min, culture temperature 30℃ , culture period 5 days. Under the optimized conditions, the β-glucosidase activity reached 44.11 IU/mL, with the improvement of 36% compared with the original activity of 32.87 IU/mL.%本研究对Aspergillus niger Glu05生产β-葡萄糖苷酶的培养基组分及培养条件进行了优化.优化后的培养基组成和培养条件分别为:麸皮4％,tryptone 4％,1μmol MnSO4,1μmol NaCl,KH2PO40.2％,oH自然,摇床转速250 r/min,培养温度30℃,培养周期5d.优化后发酵液中酶活力达到44.11 IU/mL,与初始的产酶水平32.87 IU/mL相比,提高了36％.