紫外光共聚交联制备GelMA/PEGDA水凝胶

摘要

为避免物理交联明胶基水凝胶的热不稳定性,以及化学方法交联明胶基水凝胶存在的毒性,本文采用丙烯酰化的方法将甲基丙烯酸酐(MA)与明胶反应,在明胶分子链上引入双键结构,并且实现了紫外光照射引发甲基丙烯酰胺基明胶(GelMA)与聚乙二醇双丙烯酸酯(PEG-DA)共聚交联制备水凝胶.研究了不同的MA加入量对明胶修饰度的影响,并对GelMA/PEGDA交联水凝胶理化性质进行了测试和分析.结果表明:体系中PEGDA含量增加,能释放更多的自由基,增加交联反应的活性和程度,使水凝胶形成更加致密的三维网络结构.并且GelMA/PEGDA交联水凝胶在37℃比GelMA交联水凝胶更加稳定.GelMA/PEGDA交联水凝胶将来有望成为组织工程的支架材料.%To avoid the thermolability of physically cross-linked gelatin hydrogel and toxicity of chemically crosslinked gelatin hydrogel,double bonds were introduced to gelatin molecule chain by acrylation,and then gelatin methacrylamide(GelMA) and polyethylence glycol diacrylate(PEGDA) were cross-linked to hydrogels by the UV photopolymerization.The relationship between the quantity of methacrylic anhydride(MA) and degree of modification and physicochemical properties of GelMA/PEGDA were investigated and analyzed.The result indicated that more radicals could be released in the system when more PEGDA were added.So the activity and extent of crosslinking reaction was increased and hydrogel with much denser 3D network was formed.Crosslinked GelMA/PEGDA hydrogel have better thermal stability than cross linked GelMA at 37 ℃.Cross-linked GelMA/PEGDA hydrogel can be used as scaffold materials of tissue engineering.