杨树环化酶基因组织表达模式和蛋白定位

摘要

Semi-quantitative RT-PCR analysis showed thatPotri.006G237100 gene transcripts are very low in cambium, young leaf and apical bud ofPopulus trichocarpa, but high in xylem, petiole and root. Also, high abundance ofPotri.006G237100 gene transcripts was detected in lignifying stems. These data suggest thatPotri.006G237100 gene is speciifcally and abundantly expressed in the lignifying tissues ofP. trichocarpa. In addition, the DNA fragement ofPotri.006G237100gene was constructed into plant expression vector pGWB5 and transformed into Arabidopsis plant. Five transgenic plants were attained through molecular identiifcation. Confocal laser scanning microscope analysis suggested that Potri.006G237100-GFP fusion protein is located in the cytoplasm of transgenic plants.%半定量RT-PCR显示，毛果杨形成层、幼叶和顶芽中的Potri.006G237100转录产物极低，但在木质部、叶柄和根中其转录水平却较髙，在木质化茎节其转录产物也呈现高丰度积累，这表明Potri.006G237100在毛果杨木质化组织中特异地、高丰度转录表达。实验构建pGWB5-Potri.006G237100载体，转化拟南芥、分子鉴定得到5个过量表达转基因植株，激光共聚焦分析显示融合蛋白Potri.006G237100-GFP定位在转基因植株的细胞质。