邓恩桉茎段组织培养试验初探

摘要

Stem segments of Eucalypts dunnii were used for tissue culture in this paper.Best effect with low killing rate and up to 67.5% gain rate was obtained according to the following procedures.Firstly,semi-lignified or lignified stem segments were disinfected for 6 minutes with 0.1% Hgcl2,washed 3-4 times with sterile water,then disinfected with Hgcl2 for 2 minutes.Modified medium with MS +3% sugar +3% agar +VC 0.01 mg/L +VB20.015mg/L + BA0.5mg/L +NAA0.25mg/L was used for bud differentiation and proliferation.The multiplication coefficient was 4 times,and buds grew normally with no vitrification.The two hormone combination of 0.5mg/L IBA +0.25mg/L ABT and 0.5mg/L IBA +0.5mg/L NAA had some effects on root induction of Eucalypts dunnii.But the effects were not very good.The rooting rate was only 20% with big callus,and goblet cell didn't survive easily.%采集邓恩桉茎段进行组织培养。试验结果表明：半木质化或刚木质化的茎段用浓度为0.1%Hgcl2消毒6min,用无菌水冲洗3～4次,再用Hgcl2消毒2 min效果最好,杀伤率低,获得率高达67.5%;芽分化及增殖培养基用改良MS＋3%糖＋3%的琼脂＋VC0.01 mg∕L＋VB20.015 mg∕L＋BA0.5 mg/L＋NAA0.25 mg/L,增殖系数达4倍,芽生长正常,不产生＂玻璃化＂苗;在IBA0.5 mg/L＋ABT0.25 mg/L及IBA0.5 mg/L＋NAA0.5 mg/L两种激素组合对邓恩桉不定根诱导有一点作用,但效果不理想,生根率只有20%,愈伤头大,上杯不容易成活。