罗田板栗种质资源ISSR分子标记初步鉴定

摘要

The genetic resources of 15 chestnut varieties from Luotian county of Hubei provinces were analyzed by means of inter—simple sequence repeat (ISSR) markers. The results showed that 17 proper primers with rich polymorphism were selected from 35 ISSR primers. With these 17 primers, a total of 392 bands were amplified from the genome DNAs of the 15 varieties, among which 344 bands were polymorphism bands, with a polymorphism rate of 87.8%. 15 Luotian chestnut varieties could be divided into three categories by UPGMA cluster analysis of NTSYS 2. 10e software. And there was almost no genetic difference between ordinary Bayuehong and special Bayuehong, the genetic relationship of Meiguihong and Wukeli, Liuyuebao and Guihuaxiang was very close to each other. It provided a theoretical foundation for the identification of chestnut varieties and the efficient utilization of the germplasm resources.%试验以湖北省罗田县的15个板栗主要栽培品种为材料,利用ISSR分子标记对15个板栗品种进行初步认证及亲缘关系分析.结果表明,从35条ISSR引物中筛选出17条能够扩增出清晰、稳定条带的引物；17条引物共扩增出392条带,其中多态性条带有344条,多态性条带比率为87.8％.应用NTSYS 2.10e软件进行UPGMA法聚类分析,15个板栗品种大致被分为三类.其中普通八月红和特殊八月红基本无遗传差异,玫瑰红与乌壳栗,六月暴和桂花香亲缘关系较近.初步鉴定结果可为板栗品种鉴别、分类及种质资源的有效利用提供理论依据.