采用紫外线诱变处理与含药(百茵清)培养基驯化相结合的方法,对拮抗木霉菌株T32进行改良,获得了4株在百菌清杀菌剂2 000 mg/L浓度水平下仍能较好生长的抗性菌株,并通过测定其生长速率、产孢量、敏感性、拮抗作用以及遗传稳定性等指标,筛选出了性状优良的抗性菌株T32-5-10m.试验结果表明,经诱变的木霉菌株T32-5-10m与亲本木霉菌株T32相比较,其抗药性提高了20倍,生长速率和产孢能力均高于亲本菌株,连续转接10代,其抗药性十分稳定,且对供试3种土传病原真菌的抑制率达87.56％～89.11％.%Antagonistic Trichoderma strain T32 was improved by ultraviolet mutation and chlorothalonil medicinal cultivation. 4 chlorothalonil resistance strains which could grow well when chlorothalonil concentration was 2 000 mg/L were obtained. A resistance strain T32-5-10m with good features was screened by testing'the growth rate, spore production yield, sensitivity, counteraction, and genetic stability. The results showed that compared with parent strain T32, resistance of mutant T32-5-10m was raised by 20 times; growth rate and spore production of T32-5-10m was higher than that of T32. The medical resistance of T32-5-10m was steady after 10 successive transfer cultures, and its inhibition rate against 3 soil diseases pathogens was 87.56%~89.11%.
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