NaCl胁迫下小桐子幼苗叶片蛋白质变化分析

摘要

探究NaCl胁迫下小桐子(Jatropha curcas)幼苗叶片蛋白质的变化.以小桐子幼苗叶片为材料,用去离子水、10％的NaCl、0.2％的NaOH、70％的乙醇提取蛋白质,用SDS-聚丙烯酰胺凝胶电泳检测活性蛋白质的分布及差异.结果表明,胁迫时间12h,40、60 mmol/L NaCl胁迫小桐子,幼苗叶片的蛋白质变化最明显.%To study the change of protein in the leaf of Jatropha curcas seedlings under salt stress,protein was extracted separately from leaves of J.curcas seedlings by distilled water,10％ NaCl solution,0.2％ NaOH solution and 70％ C2H5OH solution.The distribution of the protein was detected by SDS-polyacrylamide gel electrophoresis.The result showed that the change of protein in seedling leaves was the most obvious if treated with 40 mmol/L and 60 mmol/L NaCl for 12 h.