D-甘露糖电化学阻抗生物传感器的研究

摘要

Studing carbohydrate is a new frontier in chemical/biological field because the action of lectin with carbohydrate is one important courses of life activities. An electrochemical impedance spectroscopy (EIS) biosensor for D-mannose was developed using concanavalin A (ConA) as molecular recognition element. ConA were fixed at the surface of gold disk electrode and the detection was taken with electrochemical impedance spectroscopy. The results showed that the electron transfer impedance had good linear relationship with D-mannose concentration when redox couple of [Fe(CN)6]3-/4- was used as probe.%糖类的研究已经成为生物及化学领域一个新的热点。凝集素与糖类之间的作用是生命活动中一个重要的部分。试验设计了一种比较简单的D-甘露糖电化学阻抗传感器，以刀豆凝集素为分子识别物质，共价键合法将刀豆凝集素固定到金圆盘电极的表面，用电化学阻抗法进行检测。结果表明，以[Fe(CN)6]3-/4-氧化还原电对作为探针，电子转移阻抗变化值与D-甘露糖的浓度之间呈现良好的线性关系。